Mx1^tm1.1Agsa
Targeted Allele Detail

Summary

• Symbol: Mx1^tm1.1Agsa
• Name: MX dynamin-like GTPase 1; targeted mutation 1.1, Adolfo Garcia-Sastre
• MGI ID: MGI:6275981
• Synonyms: ISRE-Reporter, Mx1^gfp
• Gene: Mx1 Location: Chr16:97248235-97264106 bp, - strand Genetic Position: Chr16, 57.46 cM, cytoband C4
• Alliance: Mx1^tm1.1Agsa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269925
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Null/knockout, Reporter)
• Inducer: interferon or polyinosinic-polycytidylic acid
• Mutation: Insertion
• Mutation details: The targeting construct is designed to insert a GFP reporter-GSGGS-OVA(229-359) fusion protein followed by an ACN cassette into exon 2 (the natural translation start site) of the gene. The ACN cassette includes a neomycin cassette, Cre recombinase and testes specific promoter (tACE) flanked by loxP sites. The tACE promoter allows expression of cre during spermatogenesis resulting in removal of the ACN cassette. (J:269925)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mx1 Mutation: 81 strains or lines available

References

• Original: J:269925 Uccellini MB, et al., ISRE-Reporter Mouse Reveals High Basal and Induced Type I IFN Responses in Inflammatory Monocytes. Cell Rep. 2018 Dec 4;25(10):2784-2796.e3
• All: 9 reference(s)