Stx4a^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Stx4a^{tm1c(EUCOMM)Hmgu}
• Name: syntaxin 4A (placental); targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6280958
• Synonyms: STX4^flox
• Gene: Stx4a Location: Chr7:127439832-127450160 bp, + strand Genetic Position: Chr7, 69.77 cM, cytoband F4
• Alliance: Stx4a^{tm1c(EUCOMM)Hmgu} page
• IMPC: Stx4a gene page

Mutation origin

• Mutant Cell Line: HEPD0610_3_G07
• Germline Transmission: Earliest citation of germline transmission: J:271234
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127442267 of Chromosome 7 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 127443092. The critical exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 5. (J:271234)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stx4a Mutation: 21 strains or lines available

References

• Original: J:271234 Bin NR, et al., Crucial Role of Postsynaptic Syntaxin 4 in Mediating Basal Neurotransmission and Synaptic Plasticity in Hippocampal CA1 Neurons. Cell Rep. 2018 Jun 5;23(10):2955-2966
• All: 1 reference(s)