Pkm^tm1.2Mgvh
Targeted Allele Detail

Summary

• Symbol: Pkm^tm1.2Mgvh
• Name: pyruvate kinase, muscle; targeted mutation 1.2, Matthew G Vander Heiden
• MGI ID: MGI:6281119
• Synonyms: Pkm2^-
• Gene: Pkm Location: Chr9:59563859-59586655 bp, + strand Genetic Position: Chr9, 32.03 cM
• Alliance: Pkm^tm1.2Mgvh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232562
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to contain an FRT-flanked neomycin cassette upstream of exon 9, and loxP sites that flank exon 10. Flp-recombinase to remove the neomycin selection cassette. The germline Cre-mediated recombination removed exon 10 which is alternatively spliced only in the Pkm2 isoform. When these mutant mice were bred to mice that express Cre recombinase, the exon 10 was deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is eliminated, while pyruvate kinase M1 isoform expression is unchanged. (J:232562)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pkm Mutation: 75 strains or lines available

References

• Original: J:232562 Dayton TL, et al., Germline loss of PKM2 promotes metabolic distress and hepatocellular carcinoma. Genes Dev. 2016 May 1;30(9):1020-33
• All: 1 reference(s)