Slc6a3^{tm1.1(flpo)Fuyu}
Targeted Allele Detail

Summary

• Symbol: Slc6a3^{tm1.1(flpo)Fuyu}
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 1.1, Fu Yu
• MGI ID: MGI:6316964
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^{tm1.1(flpo)Fuyu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340041
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc6a3^{tm1.1(flpo)Fuyu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc6a3 (MGI:94862)

• Mutation details: The DAT-ires-Flpo targeting construct was designed to insert a loxN-flanked neomycin resistance (neo) cassette followed by an internal ribosomal entry site (IRES) and a Flpo recombinase sequence downstream of the stop codon of the solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3) gene. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to CMV-cre mice to remove the neo cassette. (J:101977, J:340041)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc6a3 (mouse) Summary of all recombinase alleles driven by Slc6a3. Alliance mouse Slc6a3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

References

• Original: J:340041 Yang T, et al., Plastic and stimulus-specific coding of salient events in the central amygdala. Nature. 2023 Apr;616(7957):510-519
• All: 4 reference(s)