Tia1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tia1^{tm1b(KOMP)Wtsi}
• Name: cytotoxic granule-associated RNA binding protein 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6317355
• Gene: Tia1 Location: Chr6:86381201-86410387 bp, + strand Genetic Position: Chr6, 37.69 cM, cytoband D2
• Alliance: Tia1^{tm1b(KOMP)Wtsi} page
• IMPC: Tia1 gene page

Mutation origin

• Mutant Cell Line: BEPD0097_F6_D04
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 86395407 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 86397564. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tia1 Mutation: 68 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 2 reference(s)