Scmh1^{tm1e.1(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Scmh1^{tm1e.1(EUCOMM)Hmgu}
• Name: sex comb on midleg homolog 1; targeted mutation 1e.1, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6324158
• Gene: Scmh1 Location: Chr4:120262478-120387383 bp, + strand Genetic Position: Chr4, 56.52 cM, cytoband D1-D2
• Alliance: Scmh1^{tm1e.1(EUCOMM)Hmgu} page
• IMPC: Scmh1 gene page

Mutation origin

• Mutant Cell Line: HEPD0625_5_H07
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120319789 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre-mediated excision of the parental allele resulted in the removal of the promoter-driven neomycin selection cassette leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Scmh1 Mutation: 102 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 4 reference(s)