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Mmuttm2.1Mrb
Targeted Allele Detail
Summary
Symbol: Mmuttm2.1Mrb
Name: methylmalonyl-Coenzyme A mutase; targeted mutation 2.1, Matthias R Baumgartner
MGI ID: MGI:6343343
Synonyms: Mmuttm1.1Pg, Mutflox, Muttm1Bam
Gene: Mmut  Location: Chr17:41245576-41272879 bp, + strand  Genetic Position: Chr17, 19.55 cM, cytoband C-D
Alliance: Mmuttm2.1Mrb page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:277356
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6N
Mutation
description
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion
 
Mutation detailsA conditional ready (floxed) allele was created from the KO first allele chimeras generated using a EUCOMM vector from IKMC project 47381. The cassette for the KO first allele is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon. The critical exon 3 is thus flanked by loxP sites. This conditional ready allele was created by removing the FRT-flanked lacZ sequence , loxP site, neomycin cassette under the control of the human beta-actin promoter and SV40 polyA via flp-mediated recombination, leaving exon 3 flanked by loxP sites. For vector details, see Mmuttm1a(EUCOMM)Wtsi. (J:277356)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mmut Mutation:  34 strains or lines available
References
Original:  J:277356 Remacle N, et al., New in vitro model derived from brain-specific Mut-/- mice confirms cerebral ammonium accumulation in methylmalonic aciduria. Mol Genet Metab. 2018 Aug;124(4):266-277
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory