Mmuttm2.1Mrb
Targeted Allele Detail
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Symbol: |
Mmuttm2.1Mrb |
Name: |
methylmalonyl-Coenzyme A mutase; targeted mutation 2.1, Matthias R Baumgartner |
MGI ID: |
MGI:6343343 |
Synonyms: |
Mmuttm1.1Pg, Mutflox, Muttm1Bam |
Gene: |
Mmut Location: Chr17:41245576-41272879 bp, + strand Genetic Position: Chr17, 19.55 cM, cytoband C-D
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Alliance: |
Mmuttm2.1Mrb page
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Germline Transmission: |
Earliest citation of germline transmission:
J:277356
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
C57BL/6N
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
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Mutation details: A conditional ready (floxed) allele was created from the KO first allele chimeras generated using a EUCOMM vector from IKMC project 47381. The cassette for the KO first allele is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon. The critical exon 3 is thus flanked by loxP sites. This conditional ready allele was created by removing the FRT-flanked lacZ sequence , loxP site, neomycin cassette under the control of the human beta-actin promoter and SV40 polyA via flp-mediated recombination, leaving exon 3 flanked by loxP sites. For vector details, see Mmuttm1a(EUCOMM)Wtsi.
(J:277356)
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Original: |
J:277356 Remacle N, et al., New in vitro model derived from brain-specific Mut-/- mice confirms cerebral ammonium accumulation in methylmalonic aciduria. Mol Genet Metab. 2018 Aug;124(4):266-277 |
All: |
2 reference(s) |
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