Cd8a^em1Doi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cd8a^em1Doi
• Name: CD8 subunit alpha; endonuclease-mediated mutation 1, Christophe Benoist and Diane Mathis
• MGI ID: MGI:6386244
• Synonyms: CD8a^tdTomato
• Gene: Cd8a Location: Chr6:71350411-71356155 bp, + strand Genetic Position: Chr6, 32.14 cM, cytoband C
• Alliance: Cd8a^em1Doi page

Mutation origin

• Strain of Origin: NOD

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing is used to introduce a red fluorescent protein (tdTomato) with a 5' HA tag, an SV40 polyadenylation sequence and 3' HA tag into exon 1, following the ATG codon. The insertion of tdTomato results in a 64 bp deletion in exon 1. (J:253628)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd8a Mutation: 36 strains or lines available

References

• Original: J:253628 Mohan JF, et al., Imaging the emergence and natural progression of spontaneous autoimmune diabetes. Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):E7776-E7785
• All: 1 reference(s)