Gt(ROSA)26Sortm4(CAG-ZsGreen,-tdTomato)Bzsh
Targeted Allele Detail
|
Symbol: |
Gt(ROSA)26Sortm4(CAG-ZsGreen,-tdTomato)Bzsh |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Bin Zhou |
MGI ID: |
MGI:6386770 |
Synonyms: |
IR4 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
|
Alliance: |
Gt(ROSA)26Sortm4(CAG-ZsGreen,-tdTomato)Bzsh page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:278073
|
Parent Cell Line: |
Not Specified (ES Cell)
|
Strain of Origin: |
Not Specified
|
|
Allele Type: |
|
Targeted (Conditional ready, Reporter) |
Mutation: |
|
Insertion
|
|
|
Mutation details: A Rosa-CAG targeting vector was designed to insert cassette containing (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), rox-site, loxP-site, STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), second rox-site, ZsGreen sequence (an enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), second STOP (adapted from the polyA sequence between mT (membrane-targeted tdTomato) and mG (membrane-targeted eGFP) of pCA-mTmG), second loxP-site, tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), WPRE (woodchuck hepatitis virus posttranscriptional regulatory element)-polyA, and Frt flanked neomicyne selection cassette (CAG-rox-loxP-Stop-rox-ZsGreen-Stop-loxP-tdTomato-polyA-Frt-Neo-Frt) into the Rosa26 locus.
(J:278073)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Gt(ROSA)26Sor Mutation: |
997 strains or lines available
|
|
Original: |
J:278073 He L, et al., Enhancing the precision of genetic lineage tracing using dual recombinases. Nat Med. 2017 Dec;23(12):1488-1498 |
All: |
1 reference(s) |
|