Dpp4^{tm1.1(DPP4)Pbmj}
Targeted Allele Detail

Summary

• Symbol: Dpp4^{tm1.1(DPP4)Pbmj}
• Name: dipeptidylpeptidase 4; targeted mutation 1.1, Paul B McCray Jr
• MGI ID: MGI:6400999
• Synonyms: hDPP4-KI
• Gene: Dpp4 Location: Chr2:62160417-62242575 bp, - strand Genetic Position: Chr2, 35.85 cM, cytoband C2-D
• Alliance: Dpp4^{tm1.1(DPP4)Pbmj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242025
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Dpp4^{tm1.1(DPP4)Pbmj} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DPP4 (1803)

  Dpp4	(MGI:94919)

  expresses human exons 10-12 coding the MERS-CoV receptor

• Mutation details: Exons 10-12 (from codon I264 in exon 10 to codon V340 in exon 12) of the mouse were replaced with the corresponding human exons of 10-12 which comprise the critical Middle East respiratory syndrome coronavirus (MERS-CoV) receptor. A neomycin resistance gene flanked by FRT sites and a puromycin resistance gene flanked by F3 sites were inserted in intron 9 and intron 12, respectively. The selection markers were removed via Flp-mediated recombination. (J:242025)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dpp4 Mutation: 73 strains or lines available

References

• Original: J:242025 Li K, et al., Mouse-adapted MERS coronavirus causes lethal lung disease in human DPP4 knockin mice. Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):E3119-E3128
• All: 13 reference(s)