Gt(ROSA)26Sor^{em1(DPP4)Yowa}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(DPP4)Yowa}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Youchun Wang
• MGI ID: MGI:6403442
• Synonyms: Gt(ROSA)26Sortm1(SA-hDPP4-tdTomato), R26-hDPP4
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(DPP4)Yowa} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(DPP4)Yowa} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DPP4 (1803)

  Dpp4	(MGI:94919)

• Mutation details: CRISPR/Cas9 technology inserted the full-length human DPP4 cDNA linked to the red fluorescent protein tdTomato by an internal ribosomal entry site into the locus. Expression of the human cDNA is driven by the splice acceptor and A poly(A) sequence and a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) were added to enhance RNA stability and translation efficiency. Western blot analysis of lung and brain tissue confirmed expression, with highest expression in the lung. (J:279060)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:279060 Fan C, et al., A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV. Viruses. 2018 Aug 23;10(9)
• All: 3 reference(s)