Tg(Th-DRD2)1Jtw
Transgene Detail

Summary

• Symbol: Tg(Th-DRD2)1Jtw
• Name: transgene insertion 1, John Williams
• MGI ID: MGI:6405725
• Synonyms: TH-hD2S
• Transgene: Tg(Th-DRD2)1Jtw Location: unknown
• Alliance: Tg(Th-DRD2)1Jtw page

Transgene origin

• Strain of Origin: 129/Sv

Transgene description

• Transgene Type: Transgenic (Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Th-DRD2)1Jtw expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DRD2 (1813)

  Drd2	(MGI:94924)

  carries a FLAG epitope

• Tg(Th-DRD2)1Jtw expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Th (25085)

  Th	(MGI:98735)

• Mutation details: The human D2 dopamine receptor (DRD2) short isoform (hD₂S) cDNA with an amino terminus Flag epitope is expressed from the rat tyrosine hydroxylase (Th) promoter and rabbit beta-globin splice donor/acceptor sites. The transgene consists of an 8.5 kb genomic fragment from the rat tyrosine hydroxylase gene (Th) containing 5' regulatory sequences, the basal promoter, and 26 base pairs from the 5' untranslated region in exon 1 followed by a 0.7 kb cassette containing intron 2 and splice donor/acceptor sites from the rabbit beta-globin gene. The DRD2 construct consists of a consensus Kozak sequence, a signal peptide from the hemagglutinin influenza followed by the sequence for the FLAG epitope, a full-length cDNA for the DRD2 containing 1.4 kb of coding sequence and 1.0 kb of the 3' untranslated region, and the bovine growth hormone polyA sequence. (J:201702)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:201702 Gantz SC, et al., Spontaneous inhibitory synaptic currents mediated by a G protein-coupled receptor. Neuron. 2013 Jun 5;78(5):807-12
• All: 1 reference(s)