Synb^tm2.1Ics
Targeted Allele Detail

Summary

• Symbol: Synb^tm2.1Ics
• Name: syncytin b; targeted mutation 2.1, Mouse Clinical Institute
• MGI ID: MGI:6406921
• Gene: Synb Location: Chr14:69526793-69531748 bp, - strand Genetic Position: Chr14, 36.04 cM
• Alliance: Synb^tm2.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: P1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The wild-type gene was replaced by the same gene carrying a K14R point mutation in the ISU domain. The targeting vector contained a 4.3-kb 5-prime arm and a 5-kb 3-prime arm corresponding to sequences bracketing the syncytin-B ORF, a 2.6 kb fragment containing the syncytin-B unique ORF into which the K14R mutation was introduced and the neomycin resistance (neo) genes. The ORF and neo gene were flanked by FRT and LoxP recombination sites, allowing their conditional excision. In the recombinant mouse strain obtained, the FRT-flanked neo selection marker was removed by breeding with flp recombinase-expressing mice; the syncytin-B K14R mutant gene is flanked by LoxP recombination sites, allowing its conditional excision. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Synb Mutation: 7 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)