Slc20a2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc20a2^{tm1c(EUCOMM)Wtsi}
• Name: solute carrier family 20, member 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6416079
• Synonyms: Pit2^fl
• Gene: Slc20a2 Location: Chr8:22966804-23059628 bp, + strand Genetic Position: Chr8, 11.42 cM
• Alliance: Slc20a2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Slc20a2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287268
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 23029908 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 23030738. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc20a2 Mutation: 104 strains or lines available

References

• Original: J:287268 Chande S, et al., Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival. Sci Rep. 2020 Feb 20;10(1):3069
• All: 2 reference(s)