About   Help   FAQ
Gt(ROSA)26Sorem1.1(CAG-Rpl22,-Uprt,-mKate2,-Ago2)Jaws
Endonuclease-mediated Allele Detail
Summary
Symbol: Gt(ROSA)26Sorem1.1(CAG-Rpl22,-Uprt,-mKate2,-Ago2)Jaws
Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Walker Jackson
MGI ID: MGI:6416525
Synonyms: Gt(ROSA)26Sortm1.1Jaws
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sorem1.1(CAG-Rpl22,-Uprt,-mKate2,-Ago2)Jaws page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:279059
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Mutation detailsThe Tagger transgene comprises an HA-tagged ribosomal protein L22 (Rpl22-HA, for Ribo-Tag), Uracil phosphoribosyltransferase from T.gondii (TgUPRT, for TU-Tag), mKate2 red fluorescent protein with a triple nuclear localization signal (mKate2, for Nuc-Tag), and FLAG-V5-tagged Argonaute2 (FLAG-V5-Ago2, for Ago-Tag). This cassette was placed under the control of the CAG promoter and two transcription-terminating STOP cassettes Cre-dependent (LoxP-STOP-LoxP, LSL) and Flp-dependent (Frt-NeoR-Frt, FNF). In the Tagger transgene, protein-coding cistrons were separated by a sequence encoding the Porcine Teschovirus 2A peptide (P2A) and flexible linkers (GSGSG) for improved separation. The transgene was targeted to the Rosa26 locus, including a ubiquitous CAG driver followed by Flp- (FNF, Frt-NeoR-Frt) and Cre-dependent (LSL, LoxP-STOP-LoxP) terminators (STOP cassettes). The inclusion of two terminators enables transgene activation at the intersection of cell populations expressing Cre and Flp driven by distinct promoters, to precisely target cells based on combinations of cell identity markers or activity. Tagger expression cassette (in R26LK-TK-Tagger vector) was targeted into Rosa26 safe harbor locus in V6.5 ES cells using CRISPR/Cas9 as described previously. In brief, 40 g of HTV linearized with AscI was co-electroporated with 5 g CRISPR/Cas9 nickase plasmid pX335 (Feng Zhang, Addgene plasmid #42335) encoding sgRNA-targeting the homology junction. Flp-mediated recombination removed the FRT-flanked neomyocin resistance cassette. (J:279059)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  993 strains or lines available
References
Original:  J:279059 Kaczmarczyk L, et al., Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. PLoS Biol. 2019 Aug;17(8):e3000374
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/19/2024
MGI 6.24
The Jackson Laboratory