Ace2^{em1(ACE2)Yowa}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^{em1(ACE2)Yowa}
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 1, Youchun Wang
• MGI ID: MGI:6431336
• Synonyms: hACE2, hACE2-KI/NIFDC
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^{em1(ACE2)Yowa} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology inserted the full-length human ACE2 cDNA, IRES, and tdTomato gene into exon 2 of the mouse gene. Western blot analysis confirmed strong expression of the human gene in lung and kidney and very weak expression in spleen and liver, a similar expression patter to the mouse protein. PCR confirmed the absence of the mouse gene. (J:288243)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:288243 Sun SH, A Mouse Model of SARS-CoV-2 Infection and Pathogenesis. Cell Host Microbe. 2020;20:1-10
• All: 12 reference(s)