Abcb1a^em1Sbkv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Abcb1a^em1Sbkv
• Name: ATP-binding cassette, sub-family B member 1A; endonuclease-mediated mutation 1, Sergei B Koralov
• MGI ID: MGI:6437907
• Synonyms: Abcb1a^AME, Abcb1a^ametrine
• Gene: Abcb1a Location: Chr5:8710077-8798575 bp, + strand Genetic Position: Chr5, 3.43 cM
• Alliance: Abcb1a^em1Sbkv page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing is used to replace the stop codon with a bicistronic reporter containing a P2A peptide sequence and the fluorescent reporter ametrine. In addition, an in-frame 9 nucleotide deletion is found near the 3' end of the gene. The deletion is not believed to affect protein expression. Ametrine is a long stokes shift (excitation at 406 nanometers and emission at 526 nanometers) yellow fluorescent protein derived from Aequorea victoria. (J:259930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Abcb1a Mutation: 93 strains or lines available

References

• Original: J:259930 Cao W, et al., The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids. Immunity. 2017 Dec 19;47(6):1182-1196.e10
• All: 1 reference(s)