Tlr9^em1Blev
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tlr9^em1Blev
• Name: toll-like receptor 9; endonuclease-mediated mutation 1, Beth Levine
• MGI ID: MGI:6448430
• Synonyms: TLR9-HA
• Gene: Tlr9 Location: Chr9:106099797-106104075 bp, + strand Genetic Position: Chr9, 57.46 cM
• Alliance: Tlr9^em1Blev page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: The sequence just upstream of the stop codon was targeted for insertion of HA tag sequence with a gRNA (5'-GGGACCTACAGCAGAATAG-3') and an oligonucleotide donor, using CRISPR/Cas9 technology. The resulting allele has sequence coding for nine C-terminal haemagglutinin (HA) amino-acids inserted in-frame immediately upstream of the stop codon. (J:291761)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlr9 Mutation: 65 strains or lines available

References

• Original: J:291761 Liu Y, et al., TLR9 and beclin 1 crosstalk regulates muscle AMPK activation in exercise. Nature. 2020 Feb;578(7796):605-609
• All: 1 reference(s)