Hprt1^{tm1(CAG-EGFP)Laumm}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(CAG-EGFP)Laumm}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Laura M Machesky
• MGI ID: MGI:6459291
• Synonyms: Lifeact-mEGFP
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(CAG-EGFP)Laumm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275378
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The following sequences were inserted in place of the endogenous promoter, exons 1-2 and 5' end of intron 2, which are lacking in the Hprt^b-m3 allele-containing ES cels: the CAG promoter, a loxP site flanked STOP cassette, sequence coding for a chimeric peptide joining the 17-amino acid F-actin-binding Lifeact peptide to monomeric EGFP, poly(A) signal sequence and human HPRT promoter and exon 1 and mouse Hprt intron 1, exon 2 and part of intron 2. Mice carrying this allele will only express the Lifact-EGFP fusion protein when crossed with mice expressing a cre recombinase allele. (J:275378)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:275378 Schachtner H, et al., Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. Eur J Cell Biol. 2012 Nov-Dec;91(11-12):923-929
• All: 4 reference(s)