Mecr^{tm1(fabI)Akast}
Targeted Allele Detail

Summary

• Symbol: Mecr^{tm1(fabI)Akast}
• Name: mitochondrial trans-2-enoyl-CoA reductase; targeted mutation 1, Alexander J Kastaniotis
• MGI ID: MGI:6468551
• Gene: Mecr Location: Chr4:131570781-131595097 bp, + strand Genetic Position: Chr4, 64.52 cM
• Alliance: Mecr^{tm1(fabI)Akast} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A sequence encoding the bacterial fabI ETR (2-enoyl-thioester reductase), a functional prokaryotic ortholog of Mecr, was introduced into exon 2 of the mouse Mecr gene. The construct was C-terminally tagged with a double HA epitope tag. The splicing acceptor sites of exon 2 and 54 nucleotides of the exon 2 itself were maintained in-frame with the fabI coding region. The Mecr mitochondrial targeting sequence (MTS) is encoded by exon 1. These alterations are predicted to yield a chimeric mRNA encoding HA-tagged fabI N-terminally extended with the Mecr MTS. The construct was followed by the strong transcriptional terminator BGH attached to an FRT-flanked selection cassette to exclude the possibility of producing intact Mecr. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mecr Mutation: 17 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)