Ace2^{tm1(ACE2)Dwnt}
Targeted Allele Detail

Summary

• Symbol: Ace2^{tm1(ACE2)Dwnt}
• Name: angiotensin converting enzyme 2; targeted mutation 1, David Wentworth
• MGI ID: MGI:6469881
• Synonyms: hACE2 KI
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^{tm1(ACE2)Dwnt} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296356
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Ace2^{tm1(ACE2)Dwnt} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ACE2 (59272)

  Ace2	(MGI:1917258)

• Mutation details: The targeting construct is designed to replace exon 2 with a human ACE2 cDNA inserted in-frame with the endogenous initiation codon. in addition, a loxP site is placed upstream of the ACE2 cDNA and a FRT-flanked neomycin resistance (neo) cassette and a second loxP site is placed downstream of the ACE2 cDNA. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:296356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:296356 Zhou B, et al., SARS-CoV-2 spike D614G variant confers enhanced replication and transmissibility. bioRxiv. 2020
• All: 12 reference(s)