Gt(ROSA)26Sor^{em1(CAG-venus,-mCherry,-mCerulean)Okn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-venus,-mCherry,-mCerulean)Okn}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Hideyuki Okano
• MGI ID: MGI:6471897
• Synonyms: ROSA26^MultiFPs
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-venus,-mCherry,-mCerulean)Okn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284134
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NCrlCrlj

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced at the locus a CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), bacteriophage P1 loxP sites, Streptomyces lividans attB site, P1-like transducing phage D6 rox sites, Streptomyces alboniger Puromycin resistance gene, jellyfish GFP variant (Venus) cDNA, Entacmaea quadricolor RFP variant (mCherry) cDNA, SV40 polyA, Streptomyces lividans attP site, jellyfish GFP variant (mCerulean)cDNA, bovine growth hormone polyA, and Saccharomyces cerevisia FRT site. (J:284134)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

ES cells = B6NJ-22(UTR) (C57BL/6NCrlCrlj)

References

• Original: J:284134 Serizawa T, et al., Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Development. 2019 Nov 4;146(21):dev174938
• All: 2 reference(s)