Igh^em1Rcas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igh^em1Rcas
• Name: immunoglobulin heavy chain complex; endonuclease-mediated mutation 1, Rafael Casellas
• MGI ID: MGI:6725128
• Synonyms: nanomouse
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/cas9 genome editing is used to delete the mouse CH1 (constant heavy chain) exons and replace the mouse VH (variable heavy chain) portion of Igh with a VHH minigene containing: mouse Vh promoter (containing octamer and TATA box, 250 bps), mouse leader exons-intron (150 bp), camelid VHH open reading frame (300 bps), and mouse downstream sequences containing recombination sequences (100 bps). The 15 kD VHH (Variable Heavy chain domains of a Heavy chain or nanobodies [Nbs]) minigene contains 18 alpaca, 7 dromedary and 5 bactrian camel VHH genes in a 25 kB insertion. Each gene is fused to a VH promoter, leader exons and recombination signal sequences. (J:307753)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:307753 Xu J, et al., Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants. Nature. 2021 Jun 7
• All: 1 reference(s)