Tg(Pitx3-IFP,-tdTomato,-Citrine)2Past
Transgene Detail

Summary

• Symbol: Tg(Pitx3-IFP,-tdTomato,-Citrine)2Past
• Name: transgene insertion 2, Jeroen Pasterkamp
• MGI ID: MGI:6726603
• Synonyms: Pitx3-ITC
• Transgene: Tg(Pitx3-IFP,-tdTomato,-Citrine)2Past Location: unknown
• Alliance: Tg(Pitx3-IFP,-tdTomato,-Citrine)2Past page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The transgene consists of mouse Pitx3 BAC clone RP23-125F3 with a ITC cassette inserted into the ATG start codon in exon 2 of the Pitx3 gene. The ITC cassette has the following components in order, FRT, loxP, IFP(infrared fluorescent protein)-polyA, loxP, tdTomato-polyA, FRT, Citrine-polyA, with each gene followed by a stop signal that prevents expression of the next gene. Without recombination, IFP is expressed. While Cre/loxP recombination will express tdTomato and Flp/frt recombination will express Citrine. Founder information and line numbers were not available. Different founders displayed comparable labeling patterns. Line 2 was used for the study. (J:297714)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:297714 Brignani S, et al., Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development. Neuron. 2020 Aug 19;107(4):684-702.e9
• All: 2 reference(s)