Ace2^em1Guru
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^em1Guru
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 1, Channabasavaiah Gurumurthy
• MGI ID: MGI:6763023
• Synonyms: mAce2^{KI-31K,82M,353K}
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^em1Guru page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to insert 5 amino acid substitutions. The amino acids 30, 31, 34 in exon 2 were changed from asparagine to glutamic acid (N30D), asparagine to lysine (N31K), and glutamine to aspartic acid (Q34D) respectively, amino acid 82 in exon 3 was changed from serine to methionine (S82M), and the amino acid 353 in exon 9 was changed from histidine to lysine (H353K). The mutations are designed to completely humanize the mouse Ace2 coding sequence in a region important for binding to the SARS-CoV-2 spike protein. (J:298750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:298750 Gurumurthy CB, et al., Genetically modified mouse models to help fight COVID-19. Nat Protoc. 2020 Dec;15(12):3777-3787
• All: 1 reference(s)