Ace2^{em1(ACE2)Mlkn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^{em1(ACE2)Mlkn}
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 1, Mark L Kahn
• MGI ID: MGI:6842318
• Synonyms: hACE2^fl
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^{em1(ACE2)Mlkn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:315719
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Ace2^{em1(ACE2)Mlkn} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ACE2 (59272)

  Ace2	(MGI:1917258)

• Mutation details: CRISPR/cas9 meditated recombination inserted a cassette containing a modified human ACE2 cDNA, loxP site, woodchuck hepatitis virus post-transcriptional regulatory element, simian virus 40 polyA sequence, loxP site, and an FRT-flanked PGK-neo and loxP site directly after the ATG site in exon 2. The human ACE2 cDNA was modified to use the mouse Ace2 signal peptide and codon-optimized for a mouse. (J:315719)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:315719 Tang AT, et al., SARS-CoV-2 infection olfactory cells and neurons drives acute lung injury and lethal COVID-19 in mice. bioRxiv. 2021 Dec
• All: 3 reference(s)