Ace2^{em2(ACE2/GFP)Mlkn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^{em2(ACE2/GFP)Mlkn}
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 2, Mark L Kahn
• MGI ID: MGI:6842319
• Synonyms: hACE2-GFP^fl
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^{em2(ACE2/GFP)Mlkn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:315719
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
• Mutation: Insertion
• Ace2^{em2(ACE2/GFP)Mlkn} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ACE2 (59272)

  Ace2	(MGI:1917258)

• Mutation details: CRISPR/cas9 meditated recombination inserted a cassette containing a modified human ACE2 cDNA fused to an monomeric-Emerald GFP reported gene, loxP site, woodchuck hepatitis virus post-transcriptional regulatory element, simian virus 40 polyA sequence, loxP site, and an FRT-flanked PGK-neo and loxP site directly after the ATG site in exon 2. The human ACE2 cDNA was modified to use the mouse Ace2 signal peptide and codon optimized for mouse. (J:315719)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:315719 Tang AT, et al., SARS-CoV-2 infection olfactory cells and neurons drives acute lung injury and lethal COVID-19 in mice. bioRxiv. 2021 Dec
• All: 1 reference(s)