Tg(Chrna6/GFP)4Lst
Transgene Detail

Summary

• Symbol: Tg(Chrna6/GFP)4Lst
• Name: transgene insertion 4, Henry A Lester
• MGI ID: MGI:6850678
• Synonyms: alph6-GFP, Tg(Chrna6*)4Lst
• Transgene: Tg(Chrna6/GFP)4Lst Location: unknown
• Alliance: Tg(Chrna6/GFP)4Lst page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Chrna6/GFP)4Lst expresses 3 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Chrna6 (MGI:106213)
  mouse	Poteg (MGI:1918202)
  mouse	Tex24 (MGI:1921539)

• Mutation details: The transgene contains BAC RP24-149l12 with a GFP reporter gene inserted into exon 5. Line 4 contains 6 copies of the transgene. (J:304873)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The copy of Chrnb3 contained within the BAC was silenced using homologous recombination replacing exon 1 with an ampicillin cassette. The BAC also contains Poteg and Tex24, which are not expressed in the brain.

References

• Original: J:304873 Mackey ED, et al., alpha6* nicotinic acetylcholine receptor expression and function in a visual salience circuit. J Neurosci. 2012 Jul 25;32(30):10226-37
• All: 7 reference(s)