Gt(ROSA)26Sor^{tm2(CAG-Foxo1*)Mschu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-Foxo1*)Mschu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Markus Schubert
• MGI ID: MGI:6861843
• Synonyms: FoxO1DN
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-Foxo1*)Mschu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301891
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The CAG-a loxP site flanked neo-Westphal stop-FLAG-mouse Foxo1* cassette followed by an FRT site-framed IRESeGFP expression cassette were inserted into ROSA26 locus via homologous recombination in the ES cells. The Foxo1 cDNA with deleted transactivation domain acting as dominant-negative Foxo1 transcripts. In presence of cre recombinase, a loxP site flanked neo-Westphal stop sequence is removed to allow expression of mutated Foxo1 gene driven by CAG promoter. (J:301891)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:301891 Stohr O, et al., Insulin receptor signaling mediates APP processing and beta-amyloid accumulation without altering survival in a transgenic mouse model of Alzheimer's disease. Age (Dordr). 2013 Feb;35(1):83-101
• All: 2 reference(s)