Adgrl2^tm1.2Sud
Targeted Allele Detail

Summary

• Symbol: Adgrl2^tm1.2Sud
• Name: adhesion G protein-coupled receptor L2; targeted mutation 1.2, Thomas C Sudhof
• MGI ID: MGI:7262996
• Synonyms: Lphn2^fl, Lphn^cKO, Lphn^loxP
• Gene: Adgrl2 Location: Chr3:148521219-148696191 bp, - strand Genetic Position: Chr3, 74.1 cM
• Alliance: Adgrl2^tm1.2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246190
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac x C57BL/6J

Mutation description

• Allele Type: Targeted (No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to introduce a loxP site upstream of the first coding exon (exon 2). A segment carrying an F1 FRT site, FRT site, 1 kb of the mouse engrailed splice acceptor, 5 kb of Adgrl2 cDNA fused to mVenus green fluorescent protein, a polyA signal, FRT site, PGK-neomycin resistance cassette, tandem FRT and F1 FRT sites, and a loxP site were placed in intron 2. Flp-mediated recombination in the germline resulted in the removal of the cDNA/Venus gene fusion and the neomycin selection cassette, leaving loxP sites flanking exon 1. (J:246190)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adgrl2 Mutation: 81 strains or lines available

References

• Original: J:246190 Anderson GR, et al., Postsynaptic adhesion GPCR latrophilin-2 mediates target recognition in entorhinal-hippocampal synapse assembly. J Cell Biol. 2017 Nov 06;216(11):3831-3846
• All: 3 reference(s)