Gt(ROSA)26Sor^{em3(CAG-Venus*)Rkuhn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em3(CAG-Venus*)Rkuhn}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 3, Ralf Kuhn
• MGI ID: MGI:7286320
• Synonyms: GER10, R26-GFP_KI-ABE
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em3(CAG-Venus*)Rkuhn} page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed to insert the ABE (Adenine Base Editor) reporter construct into the Gt(ROSA)26Sor locus containing a CAG promoter, mutated Venus*, bovine hGH, and polyA sequence. Venus* is a non-functional reporter that has codon 81 mutated into a stop codon. When an ABE together with a guide RNA that binds at the mutant site is introduced into cells, the stop is converted into a glutamine codon and the reporter will express GFP. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)