Atp5if1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Atp5if1^{tm1c(EUCOMM)Wtsi}
• Name: ATP synthase inhibitory factor subunit 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7287490
• Synonyms: ATPIF1 flox
• Gene: Atp5if1 Location: Chr4:132257866-132260970 bp, - strand Genetic Position: Chr4, 65.51 cM
• Alliance: Atp5if1^{tm1c(EUCOMM)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0158_4_B04
• Germline Transmission: Earliest citation of germline transmission: J:325668
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 132257577 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 132258555. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. (J:325668)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Atp5if1 Mutation: 22 strains or lines available

References

• Original: J:325668 Zhou B, et al., Upregulation of mitochondrial ATPase inhibitory factor 1 (ATPIF1) mediates increased glycolysis in mouse hearts. J Clin Invest. 2022 May 16;132(10):e155333
• All: 5 reference(s)