Foxm1^em1Pra
Endonuclease-mediated Allele Detail

Summary

• Symbol: Foxm1^em1Pra
• Name: forkhead box M1; endonuclease-mediated mutation 1, Pradip Raychaudhuri
• MGI ID: MGI:7331325
• Synonyms: FoxM1DD
• Gene: Foxm1 Location: Chr6:128339957-128352849 bp, + strand Genetic Position: Chr6, 62.98 cM
• Alliance: Foxm1^em1Pra page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Nucleotide substitutions
• Mutation details: Using CRISPR/Cas9 technology, the point mutations were introduced to change the transcription activator region of the Foxm1 gene corresponding residues in the mouse genome to aspartic acid. A phosphomimetic mutant, FoxM1DD, in which the two Plk1 phosphorylation sites were replaced by aspartic acid, retains the transcriptional activator function but fails to repress the mammary differentiation genes Gata3 and FoxA1. The founder 10 containing the mutations, FoxM1S727 mutated to aspartic acid where S727 corresponds to human S715, and FoxM1S736 mutated to aspartic acid where S736 corresponds to human S724, were used for the study. (J:326291)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxm1 Mutation: 26 strains or lines available

References

• Original: J:326291 Kopanja D, et al., Transcriptional Repression by FoxM1 Suppresses Tumor Differentiation and Promotes Metastasis of Breast Cancer. Cancer Res. 2022 Jul 5;82(13):2458-2471
• All: 1 reference(s)