Sparc^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sparc^{tm1c(EUCOMM)Wtsi}
• Name: secreted acidic cysteine rich glycoprotein; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7336234
• Synonyms: Sparc^fl
• Gene: Sparc Location: Chr11:55284985-55310906 bp, - strand Genetic Position: Chr11, 33.04 cM, cytoband B1
• Alliance: Sparc^{tm1c(EUCOMM)Wtsi} page
• IMPC: Sparc gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328359
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_st0 cassette was inserted at position 55297047 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 55297676. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:328359)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sparc Mutation: 76 strains or lines available

References

• Original: J:328359 Ryu S, et al., The matricellular protein SPARC induces inflammatory interferon-response in macrophages during aging. Immunity. 2022 Aug 10
• All: 2 reference(s)