Tigar^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tigar^{tm1c(EUCOMM)Wtsi}
• Name: Trp53 induced glycolysis regulatory phosphatase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7424999
• Synonyms: Tigar^fl
• Gene: Tigar Location: Chr6:127062079-127086520 bp, - strand Genetic Position: Chr6, 61.92 cM
• Alliance: Tigar^{tm1c(EUCOMM)Wtsi} page
• IMPC: Tigar gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324752
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127069049 of Chromosome 6 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 127070460. The critical exon 3 is thus flanked by loxP sites. The lacZ and neo genes were removed via flp-mediated recombination generating an exon 3 floxed conditional ready allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:324752)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tigar Mutation: 56 strains or lines available

References

• Original: J:324752 Tang Y, et al., TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling. Elife. 2022 Mar 7;11:e73360
• All: 1 reference(s)