Gt(ROSA)26Sor^{tm2.1(CAG-Prkag1)Dca}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-Prkag1)Dca}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, David Carling
• MGI ID: MGI:7431422
• Synonyms: WT-Tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-Prkag1)Dca} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251124
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A CAGGS promoter, floxed stop cassette, cDNA encoding wild-type mouse Prkag1, and a sequence encoding the Flag epitope at the C terminus were inserted via homologous recombination into the Gt(ROSA)26Sor locus. The neo-selection cassette was removed by crossing to FlpO-expressing mice. (J:251124)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:251124 Woods A, et al., Liver-Specific Activation of AMPK Prevents Steatosis on a High-Fructose Diet. Cell Rep. 2017 Mar 28;18(13):3043-3051
• All: 2 reference(s)