Kras^tm5.1Cmco
Targeted Allele Detail

Summary

• Symbol: Kras^tm5.1Cmco
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 5.1, Christopher M Counter
• MGI ID: MGI:7432938
• Synonyms: Kras^LSL-comG12D
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm5.1Cmco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328899
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Single point mutation
• Mutation details: Exons 2-4 were replaced by a cassette consisting of a transcriptional repressor sequence (STOP) flanked by loxP sites, cDNA sequence for the first three coding exons (2-4) with 93 rare codons converted to the most commonly used codons in the mouse genome and including the p.G12D oncogenic mutation, followed by the 5' 564 bp of intron 4, and an FRT site flanked neomycin resistance gene cassette. The neo cassette was removed via Flp-mediated recombination. Removal of the stop cassette is required to get expression of the mutant Kras. (J:328899)

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm5.1Cmco

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 90 strains or lines available

References

• Original: J:328899 Le Roux O, et al., Genetically manipulating endogenous Kras levels and oncogenic mutations in vivo influences tissue patterning of murine tumorigenesis. Elife. 2022 Sep 7;11:e75715
• All: 2 reference(s)