Ube3b^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ube3b^{tm1d(EUCOMM)Hmgu}
• Name: ubiquitin protein ligase E3B; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7442445
• Synonyms: Ube3b^-
• Gene: Ube3b Location: Chr5:114518668-114559230 bp, + strand Genetic Position: Chr5, 55.99 cM
• Alliance: Ube3b^{tm1d(EUCOMM)Hmgu} page
• IMPC: Ube3b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333720
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 114527986 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 114528771. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333720)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube3b Mutation: 70 strains or lines available

References

• Original: J:333720 Ambrozkiewicz MC, et al., The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc. Mol Psychiatry. 2021 Jun;26(6):1980-1995
• All: 1 reference(s)