Ambra1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ambra1^{tm1c(EUCOMM)Wtsi}
• Name: autophagy/beclin 1 regulator 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7448481
• Synonyms: Ambra1^fl
• Gene: Ambra1 Location: Chr2:91560479-91749194 bp, + strand Genetic Position: Chr2, 50.63 cM
• Alliance: Ambra1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ambra1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330579
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91597600 of Chromosome 2 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 91598494. The critical exon 4 is thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination generating a conditional exon 4 floxed allele. (J:330579)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ambra1 Mutation: 118 strains or lines available

References

• Original: J:330579 Gambarotto L, et al., Ambra1 deficiency impairs mitophagy in skeletal muscle. J Cachexia Sarcopenia Muscle. 2022 Aug;13(4):2211-2224
• All: 1 reference(s)