Col1a2^{em3(cre/ERT2)Bzsh}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Col1a2^{em3(cre/ERT2)Bzsh}
• Name: collagen, type I, alpha 2; endonuclease-mediated mutation 3, Bin Zhou
• MGI ID: MGI:7451377
• Synonyms: Col1a2-CreER
• Gene: Col1a2 Location: Chr6:4505618-4541543 bp, + strand Genetic Position: Chr6, 1.81 cM
• Alliance: Col1a2^{em3(cre/ERT2)Bzsh} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Col1a2^{em3(cre/ERT2)Bzsh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Col1a2 (MGI:88468)

• Mutation details: Using the CRISPR/Cas9 technology, a cDNA encoding cre/ERT2 followed by the Woodchuck Post-transcriptional regulatory element and a polyadenylation sequence was inserted in frame with the translational start codon of the Col1a2 gene. (J:309164)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Col1a2 (mouse) Summary of all recombinase alleles driven by Col1a2. Alliance mouse Col1a2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a2 Mutation: 119 strains or lines available

References

• Original: J:309164 Tian X, et al., Generation of a self-cleaved inducible Cre recombinase for efficient temporal genetic manipulation. EMBO J. 2020 Feb 17;39(4):e102675
• All: 2 reference(s)