Ace2^em2Mccc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^em2Mccc
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 2, Maria Cecilia C Canesso
• MGI ID: MGI:7468714
• Synonyms: Ace2^TM, Ace2^TripleMutant
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^em2Mccc page

Mutation origin

• Strain of Origin: C57BL/6J-Ace2^em1Mccc

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology introduced a serine to methionine substitution at amino acid 82 and a phenylalanine to tyrosine substitution at amino acid 83 (p.S82M/F83Y) into the Ace2^em1 zygotes containing the p.H353K change. The Y83 residue in human makes multiple direct contacts with the SARS-CoV-2 RBD. The neighboring position was also humanized due to proximity to F83 and the nonconservative nature of the replacement. These three amino acid substitutions are sufficient to allow infection of mice with multiple strains of SARS-CoV-2. (J:335150)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:335150 Nakandakari-Higa S, et al., A minimally-edited mouse model for infection with multiple SARS-CoV-2 strains. Front Immunol. 2022;13:1007080
• All: 1 reference(s)