2210408F21Rik^em1Liuq
Endonuclease-mediated Allele Detail

Summary

• Symbol: 2210408F21Rik^em1Liuq
• Name: RIKEN cDNA 2210408F21 gene; endonuclease-mediated mutation 1, Liuqing Yang
• MGI ID: MGI:7488539
• Synonyms: Pair^-
• Gene: 2210408F21Rik Location: Chr6:31197286-31314330 bp, + strand Genetic Position: Chr6, 12.56 cM
• Alliance: 2210408F21Rik^em1Liuq page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: Two point mutations, chr6:31197455G>A and chr6:31197457A>T (GRCm39), were created using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutations in intron 1 eliminate the exon 1 splice donor site, changing it from from G-GTA to G-ATT, which virtually eliminates transcript expression from this allele. (J:324861)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any 2210408F21Rik Mutation: 2 strains or lines available

References

• Original: J:324861 Li Y, et al., A noncoding RNA modulator potentiates phenylalanine metabolism in mice. Science. 2021 Aug 6;373(6555):662-673
• All: 1 reference(s)