Eif2b5^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Eif2b5^{tm1c(EUCOMM)Wtsi}
• Name: eukaryotic translation initiation factor 2B, subunit 5 epsilon; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7491925
• Synonyms: Eif2b5^fl
• Gene: Eif2b5 Location: Chr16:20317567-20328073 bp, + strand Genetic Position: Chr16, 12.45 cM
• Alliance: Eif2b5^{tm1c(EUCOMM)Wtsi} page
• IMPC: Eif2b5 gene page

Mutation origin

• Mutant Cell Line: EPD0116_1_G09
• Germline Transmission: Earliest citation of germline transmission: J:282941
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 20319681 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 20321842. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:282941)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eif2b5 Mutation: 31 strains or lines available

References

• Original: J:282941 Schmidt S, et al., A MYC-GCN2-eIF2alpha negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer. Nat Cell Biol. 2019 Nov;21(11):1413-1424
• All: 1 reference(s)