Ace2^{em2(ACE2)Mlkn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^{em2(ACE2)Mlkn}
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 2, Mark L Kahn
• MGI ID: MGI:7507528
• Synonyms: hACE2^hypo
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^{em2(ACE2)Mlkn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337814
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Ace2^{em2(ACE2)Mlkn} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ACE2 (59272)

  Ace2	(MGI:1917258)

• Ace2^{em2(ACE2)Mlkn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ace2 (MGI:1917258)

• Mutation details: The coding sequence of the first translated exon of the mouse gene (exon 2) was replaced by an expression cassette containing a loxP site, a modified human ACE2 cDNA, loxP site, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 polyA signal, and FRT-flanked PGK-neomycin resistance gene via CRISPR/Cas9 technology. The human ACE2 cDNA was modified to use the mouse Ace2 signal peptide and codon-optimized for a mouseThe first loxP site interrupts the 5 untranslated sequence of the mouse Ace2. (J:337814)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 56 strains or lines available

References

• Original: J:337814 Tang AT, et al., Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection. PLoS Biol. 2023 Feb;21(2):e3001989
• All: 1 reference(s)