Car4^{em1(cre/ERT2)Jich}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Car4^{em1(cre/ERT2)Jich}
• Name: carbonic anhydrase 4; endonuclease-mediated mutation 1, Jichao Chen
• MGI ID: MGI:7522064
• Synonyms: Car4^CreER
• Gene: Car4 Location: Chr11:84848612-84856870 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Car4^{em1(cre/ERT2)Jich} page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Car4^{em1(cre/ERT2)Jich} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Car4 (MGI:1096574)

• Mutation details: CRISPR/Cas9 technology was used to replace the start codon of the carbonic anhydrase 4 (Car4) gene with a Cre-ERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain). (J:345362)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Car4 (mouse) Summary of all recombinase alleles driven by Car4. Alliance mouse Car4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Car4 Mutation: 25 strains or lines available

References

• Original: J:345362 Hutchison V, et al., Inducible tricolor reporter mouse for parallel imaging of lysosomes, mitochondria, and microtubules. J Cell Biol. 2024 Jan 1;223(1)
• All: 1 reference(s)