Fbxw2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Fbxw2^{tm1c(EUCOMM)Hmgu}
• Name: F-box and WD-40 domain protein 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7522384
• Synonyms: Fbxw2^fl
• Gene: Fbxw2 Location: Chr2:34694526-34716323 bp, - strand Genetic Position: Chr2, 22.96 cM
• Alliance: Fbxw2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Fbxw2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339378
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 34700726 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 34701600. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:339378)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxw2 Mutation: 21 strains or lines available

References

• Original: J:339378 Yin Y, et al., The FBXW2-MSX2-SOX2 axis regulates stem cell property and drug resistance of cancer cells. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20528-20538
• All: 1 reference(s)