Fbxw2^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Fbxw2^{tm1d(EUCOMM)Hmgu}
• Name: F-box and WD-40 domain protein 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7522385
• Synonyms: Fbxw2^delta
• Gene: Fbxw2 Location: Chr2:34694526-34716323 bp, - strand Genetic Position: Chr2, 22.96 cM
• Alliance: Fbxw2^{tm1d(EUCOMM)Hmgu} page
• IMPC: Fbxw2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339378
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt1 cassette was inserted at position 34700726 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 34701600. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:339378)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxw2 Mutation: 23 strains or lines available

References

• Original: J:339378 Yin Y, et al., The FBXW2-MSX2-SOX2 axis regulates stem cell property and drug resistance of cancer cells. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20528-20538
• All: 1 reference(s)