Gt(ROSA)26Sor^{tm1(UBC-EGFP)Fcam}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(UBC-EGFP)Fcam}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Fernando Camargo
• MGI ID: MGI:7526265
• Synonyms: RA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(UBC-EGFP)Fcam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341616
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Lineage barcode, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct construct contains a EGFP reporter with a WPRE, 10 target sites, an SV40-polyA signal, and 2xHS4 insulator elements inserted into the GFP 3 UTR driven by a UBC (ubiquitin C) promoter and inserted in reverse orientation into the Gt(ROSA)26Sor locus. A "barcode" 276 bp array containing 10 target sites in a unique arrangement is inserted upstream of the EGFP polyadenylation site. (J:341616)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:341616 Li L, et al., A mouse model with high clonal barcode diversity for joint lineage, transcriptomic, and epigenomic profiling in single cells. Cell. 2023 Oct 12
• All: 1 reference(s)