Ifng^em1Kamm
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ifng^em1Kamm
• Name: interferon gamma; endonuclease-mediated mutation 1, Thomas Kammertoens
• MGI ID: MGI:7565456
• Synonyms: Ifngamma^deltaKRKR
• Gene: Ifng Location: Chr10:118276951-118281797 bp, + strand Genetic Position: Chr10, 66.75 cM
• Alliance: Ifng^em1Kamm page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Intergenic deletion
• Mutation details: CRISPR/Cas9 endonuclease-mediated genome editing is used to create a 12 base pair deletion in exon 4. The mutation removes the KRKR coding sequence. Two guide RNAs (5'-ccagcctcaggaagcg gaaa-3'; 5'-cggaatccagcctcaggaag-3'), cas9 endonuclease and a 120-nucleotide (nt) repair template are used. The deletion disrupts binding of heparin sulfate but does not affect IFNGR binding. (J:335280)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ifng Mutation: 48 strains or lines available

References

• Original: J:335280 Kemna J, et al., IFNgamma binding to extracellular matrix prevents fatal systemic toxicity. Nat Immunol. 2023 Mar;24(3):414-422
• All: 1 reference(s)