Cyp1b1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyp1b1^{tm1d(KOMP)Wtsi}
• Name: cytochrome P450, family 1, subfamily b, polypeptide 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7569044
• Gene: Cyp1b1 Location: Chr17:80014369-80022490 bp, - strand Genetic Position: Chr17, 50.3 cM
• Alliance: Cyp1b1^{tm1d(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0315_3_G08
• Germline Transmission: Earliest citation of germline transmission: J:287398
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 80020453 of Chromosome 17 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 80021951. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ sequence and neomycin resistance gene. Subsequent cre expression deleted the floxed exon 2 and generated a knockout mouse. (J:287398)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp1b1 Mutation: 26 strains or lines available

References

• Original: J:287398 Falero-Perez J, et al., Targeted deletion of Cyp1b1 in pericytes results in attenuation of retinal neovascularization and trabecular meshwork dysgenesis. Trends Dev Biol. 2019;12:1-12
• All: 2 reference(s)